Light condition, flask sealing, and cultivation time on the germination and initial in vitro development of Dendrobium nobile Lindl.

Abstract One of the obstacles for production Orchidaceae plants is seed propagation. Thus, in vitro cultivation, luminosity and type sealing bottles are factors that influence germination initial development orchids. Therefore, objective was to evaluate light condition, evaluation time Dendrobium nobile Lindl. After sowing, cultures were subjected two irradiance conditions according flasks: 7.0 µmol m-2 s-1 (threaded cap) 19.0 (plastic film) conditions: white fluorescent lamp red lamp. At 30, 60 90 days, percentage germination, survival, protocorms stage 1, 2, 3 evaluated 4. DIC used, a sub-divided plot scheme 30 days after sowing 2 x factorial at days. The highest observed when using with plastic film cover (70.33%) (70.13%). survival (100%) greatest propagules, reaching 4 verified threaded cap (9.55%).